The cytoskeleton controls the morphological and dynamic characteristics of eukaryotic cells and plays an important role in maintaining cellular integrity. CD BioSciences saves you more research time and new discoveries with one-stop protein purification services through a wealth of protein expression systems and technical resources. Design and optimize your protocols with powerful assay kits and imaging systems to obtain high-quality experimental data.
Microtubules are one of the three major types of cytoskeletons in eukaryotic cells. Together with their auxiliary proteins, they form the spindle that separates chromosomes during mitosis, and it is the most important structure in all eukaryotic cell divisions. Microtubule is a spherical molecule with two types: α-tubulin and β-tubulin, which account for about 80%-95% of total microtubule, have similar three-dimensional structures and can tightly bind into dimers that serve as subunits for microtubule assembly.
The microtubule cytoskeleton in various cell [1].
Analysis of nuclear actin localization [2].
Actin is a class of microfilament-forming globular multifunctional proteins that play an important role in muscle movement as a structural protein. Actin is usually found in the filaments of transverse muscle myogenic fibers, but also in smooth muscle, and is generally available in two forms: monomeric globules of G-actin and polymeric filaments of F-actin.
Intermediate filaments are fibers between thick and thin myofilaments, widely found in eukaryotic cells, first found in smooth muscle cells, and are one of the three cytoskeletal proteins that are tough. Intermediate fibrillar proteins are basically assembled into intermediate filaments after synthesis, with few free monomers. Through cell junctions, intermediate filaments link neighboring cells together.
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct [3].
Protein expression is a molecular biology technique in which exogenous gene proteins are expressed in model organisms such as bacteria, yeast, animal cells or plant cells. It occupies a central position in genetic engineering technology.
CD BioSciences has a wide range of prokaryotic expression vectors and eukaryotic expression vectors that can be used with a variety of expression hosts. Depending on the needs of our customers, we choose expression vectors and expression hosts with different fusion tag proteins, resistance, and action elements to truly customize cytoskeletal proteins for our customers.
Customers can choose different nature protein tags according to the purpose of the experiment. Commonly used protein tags are 6xHis, Flag, GST, c-Myc, eGFP/eCFP/eYFP/mCherry, HA, SUMO, etc.
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CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
For research use only. Not intended for any clinical use.
