The analysis of various cytoskeletons and cytoskeletal proteins has a very important role in drug targeting. CD BioSciences offers a variety of analytical techniques, including flow cytometry, that help determine the role of the cytoskeleton in drug targeting and help clients analyze the role of cytoskeletal components in cellular and physiopathological processes.
Cytoskeletal Protein Analysis
The analysis of various cytoskeletal proteins has a very important role in drug targeting. Different types of analytical instruments are used to analyze cytoskeletal elements and their role in different functions and diseases. Common methods for cytoskeletal protein analysis are as follows:
- Flow Cytometry
- Indirect Immunofluorescence
- Electron Microscopy
- Sedimentation Assay
- Confocal Microscopy
- Fluorescence Spectrophotometry
- Mass Spectrometry
- Cytoblot Assay
Application of Cytoskeletal Protein Analysis
In the last decade, comparative proteomics studies have identified cytoskeletal proteins as potential biomarkers of tumor progression and metastasis. Therefore, quantitative, and qualitative analysis of cytoskeletal proteins in healthy tissues, tumors, adjacent tissues and mesenchyme is an important source of molecular information that can be used not only for tumor classification but, more importantly, for predicting patient prognosis or even the best treatment options.
- Microtubule Polymerization Analysis
The depolymerization and polymerization reaction of microtubules is one of the important models for targeted screening of antitumor compounds.
- Actin ATPase/GTPase Analysis
Myosin constitutes a large and diverse family of actin-binding proteins that hydrolyze ATP to generate force and movement. This movement contributes to muscle contraction and cell motility, which are prerequisites for cancer cell migration.
- Intermediate Filaments Functional Analysis in Cancer Metastasis
In most cancers, overexpression of intermediate filament proteins may be associated with cancer aggressiveness. Also, it has been shown that waveform protein expression is positively associated with poor prognosis in various cancers.
Our Services
The cytoskeleton is the protein contained in the cytoplasm responsible for many cellular and physiopathological events, such as maintaining cell shape, chromosome aggregation, mechanical support of cells, muscle contraction, providing mechanical strength, etc. CD BioSciences offers researchers services to visualize and analyze cytoskeletal enzyme kinetics as well as various functions with a well-established technology platform.
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
References
- Quick Q A. Microtubule-actin crosslinking factor 1 and plakins as therapeutic drug targets[J]. International Journal of Molecular Sciences, 2018, 19(2): 368.
- Wordeman L, et al. Microtubule targeting agents in disease: classic drugs, novel roles[J]. Cancers, 2021, 13(22): 5650.
For research use only. Not intended for any clinical use.