ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation. CD BioSciences offers highly sensitive ATPase/GTPase assays for the determination of ATPase/GTPase activity, providing an efficient platform for kinetic analysis of cytoskeletal enzyme activity.
Key to Energy Transport -ATPase and GTPase
The structure of ATPase [1].
ATPases, also known as adenosine triphosphatases, are a class of enzymes that catalyze the hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) and phosphate ions, a reaction that releases energy. In most cases, energy can be transferred and used to drive another chemical reaction that requires energy. This process is widely utilized by all known forms of life.
Proteins with ATPase Activity
Ion pump: sodium-potassium pump, proton pump, calcium pump, etc.
Myosin: cross-bridge.
Kinesin and dynein in axoplasmic transport.
The function of GTPase [2].
Guanosine-5'-triphosphate, (GTP), is a class of purine-like nucleoside triphosphates that serve as substrates for RNA biosynthesis during DNA transcription during DNA replication. Its structure is similar to that of the nitrogenous base guanine, with the only difference being that GTP has a ribose group attached to it and three phosphate groups, where guanine is attached to the 1-position carbon of the ribose group and the phosphate group is attached to the 5-position carbon of the ribose group.
GTPase-related Biological Processes
Energy conversion.
Gene translocation.
Microtubule polymerization and depolymerization.
Mitochondrial function.
Our Services
ATPases and GTPases are a class of enzymes that catalyze the breakdown of ATP or GTP into ADP or GDP and phosphate ions, respectively. These enzymes play a key role in microtubule kinetics, actin kinetics, and CD BioSciences offers customized cytoskeletal enzyme kinetic assays by chromatography, electrophoresis, and spectrophotometry.
- ATPase and GTPase Activity Assay for Microtubule
Several common GTPase families, such as Rac and Rho, indirectly promote microtubule assembly by facilitating GDP/GTP exchange in microtubule dimers. We measure released phosphate by binding to reporter dyes or by enzymatic conversion to reporter molecules, etc. to detect the process of ATP/GTP enzymatic reactions, such as the assay of microtubule-induced kinesin ATPase.
- Tracking ATPase/GTPase activation during cell differentiation, migration, division, and cytoskeletal rearrangement.
- Study ATPase/GTPase activation during stress fiber formation.
- Screening the effect of small molecule inhibitors/activators on ATPase/GTPase activity.
- ATPase and GTPase Activity Assay for Actin
The phosphorylation level of myosin regulatory light chain during maturation of organisms is highly correlated with actin dissociation. We offer a variety of experimental methods, including colorimetric methods, to help our customers study the effects of changes in protein phosphorylation levels on actin dissociation and actin ATPase activity.
- Dynamic measurements of ATPase and GTPase activity.
- Discovery and characterization of ATPase/GTPase inhibitors/activators.
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
References
- Chu A, et al. The V-ATPase a 3 Subunit: Structure, Function and Therapeutic Potential of an Essential Biomolecule in Osteoclastic Bone Resorption[J]. International Journal of Molecular Sciences, 2021, 22(13): 6934.
- Mosaddeghzadeh N, et al. The RHO family GTPases: mechanisms of regulation and signaling[J]. Cells, 2021, 10(7): 1831.
For research use only. Not intended for any clinical use.
