The cytoskeleton is highly dynamic in nature. With kinetic properties of polymerization and depolymerization, its arrangement is constantly undergoing active reorganization and plays an important role in the maintenance of cell morphology, cell division, signal transduction and material transport. CD BioScience provides polymerization kinetic assay services for the cytoskeleton to help clients gain a deeper understanding of the physiological functions of the cytoskeleton.
Microtubule Polymerization Dynamics
The dynamics of intracellular microtubule polymerization are under the spatiotemporal control of many microtubule-associated proteins and other mechanisms, and this regulation is central to many cellular processes. Four parameters are commonly measured to describe MT polymerization kinetics: growth rate (polymerization), shortening (depolymerization), and the frequency of transitions between these two states.
Microtubule Polymerization Analysis Method
- luorescence Assay: This method was performed by immunofluorescence staining followed by observation of microtubule morphological changes and analysis of microtubule polymerization under a laser confocal microscope.
- Absorbance Assay: The method is based on the light scattering principle, where the excitation light is scattered through the microtubes, and the degree of scattering is proportional to the concentration of the microtubular polymer in a certain range.
Actin Polymerization Dynamics
Actin polymerization provides the driving force to help with the many types of processes involved in pulling the plasma membrane into the cell, including phagocytosis, cell entry of large viruses, and endocytosis. Actin polymerization in vitro occurs in three steps: nucleation phase; elongation phase and steady state.
Our Services
Cytoskeletal polymers are generally classified into three types: actin filaments, microtubules and intermediate filaments. In eukaryotic cells these polymers work together to control cellular morphology and provide mechanical dynamics. CD BioSciences offers its clients a service to study the polymerization kinetics of the cytoskeleton by means of light scattering and fluorescent labeling.
- Polymerization Analysis of Microtubules
Microtubule-related anticancer drugs such as colchicine and paclitaxel disrupt the dynamic balance of microtubule assembly and de-assembly by affecting the polymerization-depolymerization activity of microtubule proteins. Our research platform provides two methods for microtubule aggregation analysis and helps customers to plot microtubule aggregation curves under different physiological conditions.
- Polymerization Analysis of Microfilaments
Actin microfilaments are composed mainly of evolutionarily extremely conserved actin and are a major component of the cytoskeleton. We offer a cytoskeleton analysis solution that uses a unified approach for the depolymerization/aggregation of actin microfilaments by analyzing the specific location of cytoskeletal staining markers and identifying the structure of cytoskeletal actin microfilaments followed by quantitative analysis of their length and distribution.
- Cytoskeletal Proteins Polymerization Associated Inhibitor/Activator Development
Cytoskeletal drugs often act by interfering with monomer-polymer homeostasis. We provide our clients with the most comprehensive screening of cytoskeleton-related drugs to help researchers gain a deeper understanding of the important role of cytoskeletal polymerization/depolymerization in various physiological processes.
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
Reference
- Maz P G F. CITOESQUELETO. FILAMENTOS INTERMEDIOS[J]. Morfolia, 2008, 1(1).
For research use only. Not intended for any clinical use.
In vitro polymerization of actin [1].