The cytoskeleton controls the morphological and dynamic characteristics of eukaryotic cells and plays an important role in maintaining cellular integrity. CD BioSciences use microtubule dynamic imaging, labeling and detection research tools and technical resources to save you more time for research and new discoveries. Design and optimize your protocols for high-quality experimental data using powerful assay kits and imaging systems.
Microtubule Dynamics
One of the most striking properties of microtubules is their dynamic instability. Extensive phases of microtubule growth are followed by rapid disassembly and regrowth. Microtubules grow by the addition of GTP-bound αβ-tubulin heterodimers to their ends. Incorporation into the microtubule facilitates GTP hydrolysis, resulting in the growing tip being capped by GTP-tubulin while the microtubule lattice consists primarily of GDP-tubulin. Exposing GDP-tubulin at the tip results in catastrophe, the switch from growth to shrinkage. Microtubule dynamics, therefore, are driven by the delicate balance of GTP-tubulin incorporation and rate of GTP hydrolysis.
Microtubule dynamic instability [1].
Dynamic microtubules are essential for many processes in the lives of eukaryotic cells. To study and understand the mechanisms of microtubule dynamics and regulation, in vitro reconstitution with purified components has proven a vital approach. Imaging microtubule dynamics can be instructive for a given species, isoform composition, or biochemical modification.
Unlabeled dynamic microtubules and fluorescently labeled MAPs imaged by IRM and TIRF microscopy [2].
Live Cell Imaging Technology
A very important step forward in the field of cell biology and the use of cell biology techniques is the ability to visualize cells in their state of viability. The difference between living and non-living cells is not only related to the changes that occur during life, but also during death.
| Technology |
Advantages |
Application |
| DIC |
Avoiding the toxicity of fluorescent proteins, high Resolution. |
Imaging of unstained transparent biological samples. |
| CARS |
Avoiding the toxicity of fluorescent proteins, high Resolution. |
Imaging of unstained cellular components. |
| TIRF |
High-resolution, high-quality data. |
Dynamic imaging in or near the plasma membrane. |
| FRET |
Efficient optical analysis methods for physiological activity. |
Dynamic imaging of protein interactions. |
| FRAP |
Efficient optical analysis methods for cell membrane fluidity. |
Imaging intracellular transport dynamics. |
Our Services
CD BioSciences provides the most comprehensive services for microtubule dynamic imaging projects. Through strict monitoring and effective execution, we are committed to providing the best value solutions to complete your project.
Obtaining real-time information on microtubule dynamics must ensure that the cells operate naturally during the experiment as much as possible in terms of physiological mechanisms, and therefore will make the experiment more difficult. Our high-performance imaging solutions can overcome these challenges of live cell imaging and help discover new information about cell physiology and dynamics.
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
References
- Zwetsloot A J, et al. Measuring microtubule dynamics[J]. Essays in Biochemistry, 2018, 62(6): 725-735.
- Kuo Y W, et al. In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference-Reflection Microscopy[M]/Microtubules. Humana, New York, NY, 2022: 73-91.
For research use only. Not intended for any clinical use.
