The cytoskeleton controls the morphological and dynamic characteristics of eukaryotic cells and plays an important role in maintaining cellular integrity. CD BioSciences use microtubule dynamic imaging, labeling and detection research tools and technical resources to save you more time for research and new discoveries. Design and optimize your protocols for high-quality experimental data using powerful assay kits and imaging systems.
Motor Proteins Power Cargo Transport along Cytoskeletal Filaments
Microtubules are long polymers that are usually polarized in their intrinsic structure and in their arrangement in the cell. Typically, their fast-growing positive ends extend toward the cell periphery, while their negative ends are near the center of the cell, usually anchored in the centrosome near the nucleus. This well-organized radial arrangement of filaments provides a track for the rapid transport of cargo by members of both classes of molecular motor proteins.
Microtubules Create Compartments for Organelle Transport and Interactions
Although all microtubules are polymers containing αβ microtubule dimers, not all microtubules are functionally identical. multiple isoforms of α and β microtubule subunits can combine to form a variety of molecularly distinct microtubules. The combination of various microtubule protein isoforms, post-translational modifications and MAPs gives rise to a variety of microtubules with different affinities for molecular motors. These microtubule subpopulations may act as separate compartments for the transport of specific cargoes. Restricting specific classes of cargo to microtubule subpopulations increases the likelihood that cargoes will interact and communicate, thus providing further order throughout the cytoplasm.
Peroxisomes simultaneously bind multiple microtubules or move with them in concert [1].
Microtubule-Bound Organelle Transport Imaging
Cytoskeletal elements, such as microtubules or actin filaments, play a pivotal role in regulating peptide hormone trafficking and secretion in endocrine cells. The intracellular transport of organelles driven by molecular motors along cytoskeletal fibers is a strongly regulated process that is essential for cellular function. Different organelles may exhibit different transport properties, and small perturbations to the cell can lead to profound changes in organelle transport. Therefore, in order to monitor organelle transport events in the natural state of the cell, imaging methods must meet at least two criteria: chemical selectivity and non-invasiveness.
Microtubule network properties and distance distributions [2].
Our Services
CD BioSciences provides the most comprehensive services for microtubule-bound organelle transport projects. Through strict monitoring and effective execution, we are committed to providing the best value solutions to complete your project.
With cutting-edge imaging techniques and systems, such as spinning-disk confocal microscopy and total-internal reflection fluorescence (TIRF) microscopy, we can track the labeled particles in vivo during organelle transport. We also offer a plasmonic imaging technique for tracking single organelle dynamics with nanometer precision without labels. Our professional image analysis team is proficient at analyzing and correcting transport movements, thus allowing our customers to investigate the components and function of microtubules during organelle transport.
Obtaining real-time information on microtube transportation dynamics must ensure that the cells operate naturally during the experiment as much as possible in terms of physiological mechanisms, and therefore will make the experiment more difficult. Our high-performance imaging solutions can overcome these challenges of live cell imaging and help discover new information about cell physiology and dynamics.
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
References
- Kulić I M, et al. The role of microtubule movement in bidirectional organelle transport[J]. Proceedings of the National Academy of Sciences, 2008, 105(29): 10011-10016.
- Müller A, et al. 3D FIB-SEM reconstruction of microtubule–organelle interaction in whole primary mouse β cells[J]. Journal of Cell Biology, 2021, 220(2).
For research use only. Not intended for any clinical use.
