The cytoskeleton controls the morphological and dynamic characteristics of eukaryotic cells and plays an important role in maintaining cellular integrity. CD BioSciences use intermediate filament structure imaging, labeling and detection research tools and technical resources to save you more time for research and new discoveries. Design and optimize your protocols for high-quality experimental data using powerful assay kits and imaging systems.
Discovery and Characterization of Intermediate Filament Proteins
The intermediate filament (IF) supergene family of proteins is a prevalent structural component that forms the cytoskeleton in animal tissues in a cell-type specific manner. All IF proteins display a distinctly organized, extended alpha helix conformation, which facilitates the formation of double-coiled coils, a fundamental component of these highly flexible, stress-resistant cytoskeletal filaments.
Keratin
Type I and type II keratins are intermediate filament-forming proteins expressed in epithelial cells. They are encoded by 54 evolutionarily conserved genes and are regulated in a paired and tissue type, differentiation and environment-dependent manner. Genetically determined changes in keratin coding sequences underlie highly permeable and rare diseases whose pathophysiology reflects altered cellular fragility or tissue homeostasis.
Introduction to keratin intermediate filaments (IFs) [1].
Vimentin
Intermediate filament networks and the cell nucleus [2].
The vimentin monomer has a central alpha helical structural domain covered by a non-helical amine group at the front and a carboxyl group at the end. The two monomers twist around each other to form a dimer in the shape of a convoluted spiral tube. The dynamic nature of the waveform protein is important for the flexibility of the cell. Waveform proteins are responsible for maintaining the integrity of the cytoskeleton as they provide a level of elasticity not found in microtubules and actin.
Our Services
CD BioSciences offers services including real-time intermediate filaments localization, 3D structural imaging, and crystal structure analysis by combining powerful automated image acquisition and analysis with the help of our versatile microscopic instruments. Our microscopy systems provide you with intelligent innovations in structural imaging that can help you achieve optimal image quality.
- Neurofilament Structure Imaging
Neurofilaments are a large number of fiber structures with an outer diameter of approximately 10 nm contained in neurons. It is thought to have a cytoskeletal role in maintaining neuronal morphology. We provide the most comprehensive services for neurofilament structure imaging projects.
- Amyloid Fibrils Structure Imaging Services
Amyloid fibrils are a collective term for fibrillar insoluble polypeptides formed with an overlapping beta-fold conformation. Although having a relatively small molecular size, they have a relatively well-defined structure and are also associated with numerous diseases. We provide the most comprehensive services for amyloid fibrils structure imaging projects.
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
References
- Jacob J T, et al. Types I and II keratin intermediate filaments[J]. Cold Spring Harbor perspectives in biology, 2018, 10(4): a018275.
- Patteson A E, et al. Mechanical and non‐mechanical functions of filamentous and non‐filamentous vimentin[J]. Bioessays, 2020, 42(11): 2000078.
For research use only. Not intended for any clinical use.
