Evolutionarily ancient, highly conserved actin molecules reversibly assemble into filaments that constitute one of the three major cytoskeletal polymers. Actin is involved in many cellular functions, including interactions with myosin motor proteins, intracellular transport, cell structure and motility, muscle contraction, and cytoplasmic division. CD BioSciences has become a trusted and reliable supplier to many leading international research institutions and clinical diagnostic companies by providing quality products to scientists in the life sciences worldwide. Our antibodies combine specificity, sensitivity and reproducibility to provide high quality antibody reagents for cytoskeleton research.
Micrographs of actin filament structures in cells [1].
Different Types of Actin Isoforms
Actin is the most abundant protein in most eukaryotic cells. The ability of actin to shift between monomeric (G-actin) and filamentous (F-actin) states under the control of nucleotide hydrolysis, ions and a large number of actin-binding proteins gives actin a key role in many fields.
Vertebrates express three major actin isoforms, including three α-isoforms in skeletal, cardiac, and smooth muscle and β and γ isoforms expressed in non-muscle and muscle cells.
| α-Actin |
α-actin consists of 377 amino acids, has a molecular weight of about 42 kDa, and is widely distributed in almost all muscle-type cells. This protein is mainly used to detect skeletal muscle, smooth muscle, vascular smooth muscle, cardiac muscle and myogenic tumors. |
| β-Actin |
β-actin is composed of 375 amino acids and has a molecular weight of approximately 42 kDa. Antibodies to β-actin are mainly used to label smooth muscle and tumors of its origin. β-actin protein is usually expressed at unchanged levels in cells and is therefore widely used as a reference for consistent loading of samples at Western, and is also often used for immunostaining to observe the microfilament structure of cells. |
| γ-Actin |
There is a link between γ-actin and microtubules. Inhibition of γ-actin expression leads to impaired cell migration, motility and polarity. |
We offer ready-to-use antibodies for actin, please visit our product page if you are interested.
Our Services
CD BioSciences offers a wide range of monoclonal, polyclonal and recombinant antibodies against actin proteins. We are dedicated to the development and preparation of antibodies for various applications, providing high quality antibody products and R&D services to research institutes and pharmaceutical companies.
- Monoclonal and Polyclonal Antibody Development for Actin
The use of polyclonal and monoclonal antibodies has increased significantly in recent years. Each type of antibody has its own advantages and disadvantages, and we offer efficient antibody production services, so you can choose according to your research needs.
|
Monoclonal Antibodies |
Polyclonal Antibodies |
| Preparation Cost |
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* |
| Preparation Speed |
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| Non-specific Antibodies |
* |
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| Recognition Sites |
Single Table Location |
Multiple Table Locations |
| Batch Differences |
* |
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| Stabilization |
* |
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| Advantages |
Commonly used to detect mixed proteins with high specificity. |
Usually preferred for detection of denatured proteins with higher affinity. |
- Model Organism Actin Antibody Development
We develop and produces actin antibodies for a wide range of model organisms, providing researchers with antibodies to model organism cytoskeletal targets, including human, monkey, murine, rabbit, guinea pig, zebrafish, Drosophila, and C. elegans.
- Actin Antibody Development from Different Hosts
Mice and rats are the host species most commonly used to produce research antibodies, especially in the case of monoclonal antibodies. Whereas polyclonal antibodies can be produced in mice or rats, they are usually made in larger animals, such as rabbits, goats, donkeys or sheep, from which it is easier to collect large amounts of serum.
※There are different subclasses of IgG prepared using different species of animals, and the common subclasses of IgG from different species are as follows.
| Species |
IgG Subclass |
| Rabbit |
IgG (no subclass) |
| Mouse |
IgG1, IgG2a, IgG2b, IgG2c, IgG3 |
| Rat |
IgG1, IgG2a, IgG2b, IgG2c, IgM |
| Goat |
IgG1, IgG2 |
| Caw |
IgG1, IgG2 |
| Horse |
IgGa, IgGb, IgGc, IgG(B), IgG(T) |
| Pig |
IgG1, IgG2a, IgG2b, IgG3, IgG4 |
We provide antibody development services for different hosts (mouse, rat, rabbit, goat, etc.) according to the needs of different customers and are committed to fulfill your experimental needs in all aspects.
Antibody Customization Process
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
Reference
- Pollard T D, et al. Actin, a central player in cell shape and movement[J]. science, 2009, 326(5957): 1208-1212.
For research use only. Not intended for any clinical use.
