Engineering Synthetic Biomolecular Condensates

CD BioSciences provides customized services to design a wide range of artificial disordered proteins and synthesize biomolecular condensates in bacteria and eukaryotic cells. In addition, our experts try to change the material properties of the condensates to control a variety of cellular functions and study intracellular phase behavior.

Introduction to Engineering Synthetic Biomolecular Condensates

Biomolecular condensates mediated by phase separation are a prevalent phenomenon in biological systems and provide a new framework for understanding cellular organization and synergistic-dependent cellular functions. However, the construction of artificial biomolecular condensates with specific functions remains quite challenging. Recently, with the increasing understanding of the underlying mechanisms of condensates formation and the dependence of cellular functions on the properties of such condensates, scientists can exploit the principles of macromolecular phase separation and condensate biology to develop synthetic functional biomolecular condensates, by which the benefits of phase transition and membrane-less compartments can be exploited for synthetic biology applications. Scientists have successfully utilized the pH-responsive properties of designed peptide molecules to construct biomolecular condensates in living cell lysosomes and applied them to cancer therapy.

Fig. 1. Three classes of strategies for synthetic condensate formation are shown as schematic drawings. (Nakamura H, et al., 2019)

Customized Services

Our experts have been working in the exciting field of engineered synthetic biomolecular condensates. With expertise and cutting-edge technology, CD BioSciences is committed to creating programmable synthetic biomolecular condensates designed to help you unravel the complexity of cellular processes and drive progress in a variety of fields.

Our technical team specializes in molecular design and construction strategies to design synthetic biomolecular condensates with customized properties in bacteria and eukaryotic cells. Through an understanding of the molecular interactions and mechanisms of natural condensate formation, and careful analysis of specific cellular functions or regulatory processes of interest, we offer innovative strategies to reconstruct and modify these structures in a controlled manner.

Through the careful design of synthetic components, CD BioSciences enables the spatial localization of biomolecules within cells. We can help you manipulate cellular processes, unravel complex signaling networks, and develop novel therapeutic interventions. Our engineered synthetic biomolecular condensates are widely used in the following projects:

• Design of Synthetic Biomolecular Condensates for Cellular Engineering
• Design of Synthetic Biomolecular Condensates for Metabolic Engineering

Our strategies for control synthetic biomolecular condensates:

To control the response properties and biological functions of synthetic condensates, CD BioSciences provides a number of tools to drive the formation and dissolution of biomolecular condensates via light, chemistry, or enzymes. Our strategies includes:

• By using interacting domains that actively alter the multivalency of scaffold blocks.
• Passive changes in multivalent interactions induced by chemical perturbation.
• The scaffold valence is actively changed by an enzymatic reaction.

Advantages of Our Synthetic Biomolecular Condensates

• Programmable properties that precisely control the composition and physical properties of the condensate.
• Modulates cellular functions with spatial precision.
• We offer state-of-the-art technology and computational models to tailor the properties of synthetic condensates, such as size, composition, surface properties, and internal organization, to specific experimental requirements.

The scaffold valence is actively changed by an enzymatic reactionBy providing comprehensive molecular design and construction, programmable properties, and advanced tools and techniques, CD BioSciences is able to unleash a powerful fraction of synthetic condensates and drive breakthrough discoveries in cell biology and beyond. If you have any special requirements for our services, please feel free to contact us. We are looking forward to working together on your attractive projects.