Design of Synthetic Biomolecular Condensates in Eukaryotic Cells

Eukaryotic cells compartmentalize their internal compartments to separate biochemical reactions and processes through biomolecular condensates. These biomolecular condensates are formed by liquid-liquid phase separation (LLPS). The growing knowledge of biological LLPS has led to a number of engineering efforts that provide exciting opportunities for synthetic biology. Scientists have successfully synthesized biomolecular condensates in human cells. Using peptide sequences derived from human insulin proteins, they have designed peptide molecules that form different morphological assemblies at different pH conditions and have used the acidic environment of cancer cell lysosomes to regulate the assembly of peptide molecules and achieve phase transition of peptide molecules within the lysosomes.

Fig. 1. Synthetic biomolecular condensates to engineer eukaryotic cells. (Reinkemeier CD, et al., 2021)

Customized Services

Based on knowledge of natural biomolecular condensates, our experts excel in the design and engineering of synthetic mimics of biomolecular condensates in eukaryotic cells. CD BioSciences provides comprehensive solutions for the design of synthetic biomolecular condensates in eukaryotic cells to design and manipulate these condensates to achieve the desired function and spatial organization.

Leveraging our expertise in molecular biology, biophysics, and bioengineering, we provide cutting-edge services for the precise design, construction, and functionalization of synthetic condensates tailored to specific cellular processes in eukaryotic cells.

• Customized DNA Scaffold Design
  We offer state-of-the-art computational tools and molecular modeling techniques to design customized DNA scaffolds that can be LLPS in desired cellular compartments. By precisely designing DNA sequences, our experts can tailor the properties of the coacervates, such as size, composition, and stability, to meet the specific requirements of the intended cellular function. Our synthetic biomolecular condensates mimic the natural behavior of biomolecular condensates in living cells.
• Targeting Specific Cellular Compartments
  We offer advanced targeting strategies to ensure that synthetic condensates are localized in the desired organelle, such as the nucleus, cytoplasm, or lysosome. By utilizing cellular targeting signals or specific uptake mechanisms, our synthetic condensates can be directed to the desired compartment, allowing them to perform their functional role exactly where they are needed.
• Functionalization of Bioactive Molecules
  We offer services to integrate bioactive molecules, such as enzymes, antibodies, or small molecules, into synthetic condensates. This functionalization allows the development of highly specific and targeted cellular processes, including disease diagnostics and biosensing.

The design of synthetic biomolecular condensates in eukaryotic cells represents a promising frontier in synthetic biology and cellular engineering. CD BioSciences offers comprehensive services for the engineering and manipulation of biomolecular condensates with its extensive expertise and cutting-edge services. If you have any special requirements for our services, please feel free to contact us.