Oxidative stress is frequently observed in CKD/ESRD and is a non-traditional risk factor for all causes of death. Therefore, oxidative stress has become an important diagnostic and prognostic factor and is a target for CKD prevention/treatment. High levels of oxidative stress have been identified in the early stages of CKD, which increases with progression to ESRD; and further worsens in patients with HD.
Overview of how some cytoskeletal molecules function in cellular stress [1].
Cytoskeleton in CKD Oxidative Stress
Oxidative stress is a common denominator in the pathogenesis of renal diseases, and the NADPH oxidase or mitochondrial pathway is thought to be a major source of ROS generation in the kidney, leading to podocyte damage. When podocytes are exposed to lipid radicals, they lose their F-actin fibril cytoskeletal structure and cortical rearrange filaments or form aberrant filaments with actin-rich connections. At the same time, there is a significant loss of fiber anisotropy in podocytes treated with lipid radicals, along with a loss of random orientation. These results suggest that under oxidative stress, lipid radicals may lead to abnormal podocyte formation or even dedifferentiation by affecting cytoskeletal properties and actin formation.
Lipid peroxyl radicals induce cytoskeletal rearrangements in podocytes and increase RhoA activation [2].
What We Offer
Cytoskeletal Protein Assay in Oxidative Stress
In the oxidative stress response induced by CKD, the expression and distribution of cytoskeletal proteins are affected. With rich experience in biological research, we provide our customers with cytoskeletal protein assays for different samples including serum, tissue lysate and cell lysate by ELISA and colorimetric assays. It helps our clients to study the pathological response and therapeutic development of CKD in depth.
Nephrocyte Cytoskeleton Imaging in Oxidative Stress
We detect generalized oxidative stress in cells and the morphology of the cytoskeleton under oxidative stress conditions by means of customizable fluorescent probes using conventional fluorescence microscopy, high-intensity screening, microplate fluorophotometry, or flow cytometry platforms.
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Advanced Biotechnology
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CD BioSciences provides our global customers with cost effective, high quality and efficient research solutions for cytoskeleton related diseases. We guarantee on-time delivery of our products and results, please contact us for more details.
References
- Ong M S, et al. Cytoskeletal proteins in cancer and intracellular stress: a therapeutic perspective[J]. Cancers, 2020, 12(1): 238.
- Kruger C, et al. Lipid peroxidation regulates podocyte migration and cytoskeletal structure through redox sensitive RhoA signaling[J]. Redox Biology, 2018, 16: 248-254.
For research use only. Not intended for any clinical use.
