One of the main characteristics of tumor cells is uncontrolled proliferation. In a multicellular organism, cell function and structure are closely related, so that functions related to cell growth are regulated, at least in part, by signals that can be transmitted by an organized cytoskeleton. CD BioSciences has many years of experience in providing anti-tumor screening services that target the cytoskeleton.
Anti-tumor Drug Screening Methods
- Anti-tumor Drugs Screening at Animal Level
Most of the drugs currently used in oncology chemotherapy have been discovered in animal transplantation tumor tests, making it the most used model for screening new anti-tumor drugs. After inoculation with a certain amount of tumor cells or viral tumors, a group of animals can be inoculated with the same tumor, and the growth rate is more consistent, with less individual differences, and the survival rate of inoculation is nearly 100%, so it is easy to judge the efficacy objectively.
- Anti-tumor Drugs Screening at Cell Level
Cell proliferation at the cellular level is a very important phenotype in the biomedical field, and the detection of cell proliferation is the most important test to examine the effect of drugs on tumor cells.
- CCK-8 Cell Proliferation Assay
CCK8 is a cell proliferation assay based on WST-8, which is commonly used to assess the metabolic activity of cells and is widely used for the activity of some bioactive factors, large-scale anti-tumor drug screening, cell proliferation assay, cytotoxicity assay and drug sensitivity assay, etc.
- Edu Cell Proliferation Assay
Edu cell proliferation assay can directly and accurately detect the synthesis of new DNA. Edu cell proliferation method is simple, sensitive, convenient, fast, accurate, does not require DNA denaturation, and can be labeled simultaneously with various antibodies or fluorescent proteins to detect other trait characteristics of cells.
Taxol biosynthesis schematic [1].
Cytoskeleton-targeted Anti-Cancer Drugs
All three types of cytoskeletons are chemically composed of proteins that, in cells, give them shape, help transport proteins and larger cellular components, and play a key role in the diagnosis of various diseases and in cancer therapy.
- Cytochalasins; It can bind to the positive end of microfilaments, inhibit their polymerization, cause microfilament depolymerization and inhibit various microfilament-dependent motility, and has anti-tumor potential.
- Lantrunculins: It can bind to and depolymerize microfilaments of many tumor cells, thereby inhibit the proliferation of tumor cells.
- Paclitaxel: It can bind to microtubule protein multimers to form stable microtubule bundles, alter microtubule dynamics and induce apoptosis.
- Vincristine: It can block microtubule protein aggregation into microtubules and cause rapid disintegration of intracellular microtubules, interfere with the function of spindle, and cause mitotic arrest.
- Hemiasterlin: It can bind to the vincristine site on microtubule proteins and disrupt microtubule dynamics microtubule dynamics and disrupt microtubule depolymerization.
Our Services
As cancer is one of the leading causes of human mortality, the development of anti-tumor drugs is becoming increasingly important. CD BioSciences is committed to providing biologically driven preclinical anti-tumor drug development services to our clients. These include target identification and validation, tumor model construction, and basic toxicology evaluation to advance the development of innovative oncology drugs and diagnostic candidates in the most efficient manner.
Cytoskeleton-related Anti-cancer Compound library Customization
Based on literature reports and relevant published information, we have selected a variety of compounds with known anticancer activity to form a library of anticancer activities that can be used for positive controls for specific cancers and for cancer drug development targeting the cytoskeleton. We can also customize the variety, quality, size, and arrangement of cytoskeleton-related anticancer compounds according to your needs.
Compound Library Customization Process
Cytoskeleton-related Anti-cancer Drug Development
Our drug discovery team offers a variety of cell lines, animal models, proteins and targeting assays to help you improve the efficiency of high-throughput cytoskeletal anti-cancer drug screening.
Anti-tumor Drug Screening Process
Tumor Animal Model Construction
Prepare cancer cell suspensions and inoculate animals.
Animal Model Drug Treatment
Set up drug treatment groupings and treatment methods.
Drug Efficacy Assessment
The survival time and tumor suppression of the animals
Our Advantages
Cost Effective
Customizable
High Quality
High Efficiency
CD BioSciences provides our global customers with cost effective, high quality and efficient research solutions for cytoskeleton related diseases. We guarantee on-time delivery of our products and results, please contact us for more details.
Reference
- Biggs B W, et al. Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis in Escherichia coli[J]. Proceedings of the National Academy of Sciences, 2016, 113(12): 3209-3214.
For research use only. Not intended for any clinical use.
