Actin-microtubule crosstalk is particularly important for the regulation of cell shape and polarity during cell migration and division. CD BioSciences provides a high-resolution live cell imaging platform to study cytoskeletal interactions in cell division, helping customers to investigate the regulatory mechanisms of cytoskeletal molecular networks more deeply.
Microtubule Interaction in Mitosis
The spindle is a dividing apparatus directly related to the normal separation of chromosomes formed during cell division in plants and animals. The assembly of the spindle is completed during the prophase of mitosis, and the animal cell spindle is composed of astral microtubules, interpolar microtubules, kinetochore microtubules and their binding proteins.
In both animal and plant cells, spindle bodies are formed during the prophase of mitosis. Several components of spindle bodies are microtubule proteins in their chemical nature and different microtubules interact with each other to perform this physiological process. The spindle microtubules play an important role in chromosome movement and segregation, and the kinetochore microtubules attached to the chromosomes and the assembly of the spindle are also important checkpoints in the regulation of the cell cycle.
Spindle formation in mitosis [1].
MTOCs and Centrosome-based Microtubule–Actin Interaction
Although the mitotic spindle is ultimately assembled by microtubules, however, the direct effect of microtubules on the F-actin bundle determines the location of the spindle and division planes as well as the timing of cytoplasmic division.
In mitosis, F-actin provides a capture site for the positive end of the astral microtubule to stabilize the orientation of the mitotic spindle. In concert, the F-actin network also contributes to the proper alignment and formation of the mitotic spindle and the alignment of the prometaphase chromosome.
MTOC & Centrosome Interactions [2].
Our Services
Mitosis is of vital importance in individual development and cell replacement, and normal cytoskeletal interactions guarantee the process' success. CD BioSciences has many years of experience in cytoskeletal assays, and with our technical team and instrumentation, we provide cytoskeletal interaction assays during mitosis for our global clients.
- Cytoskeletal Interaction Assay in Different Phases of Mitosis
High-resolution imaging systems enable more visual observation to study cytoskeleton interactions. We offer cytoskeleton imaging services for different cell cycles, namely G1 phase (pre-DNA synthesis), S phase (DNA synthesis), G2 phase (late DNA synthesis) and M phase (mitosis), to help you complete relevant studies more effectively.
- Different Type of Microtubules Interaction Detection in Mitosis
High-resolution imaging systems enable more visual observation to study cytoskeletal interactions. We offer imaging services for all types of important structures in the cell cycle, such as aster, centrosomes, and spindles, to help you complete your studies more effectively.
CD BioSciences has a professional team and advanced experimental equipment, and the whole process is operated by experienced technicians to provide cytoskeleton interaction assay services to our customers. If you have any needs, please contact us.
References
- McIntosh J R. Mitosis[J]. Cold Spring Harbor perspectives in biology, 2016, 8(9): a023218.
- Pimm M L, et al. New twists in actin–microtubule interactions[J]. Molecular Biology of the Cell, 2021, 32(3): 211-217.
For research use only. Not intended for any clinical use.
