Cell adhesion is essential for maintaining stable tissue structure and is a regulator of cell motility and function and has an important impact on cellular value addition and differentiation. CD BioSciences offers a variety of in vitro cell adhesion assays that can be used to determine changes in cell adhesion capacity after various drug treatments, gene editing, or changes in culture conditions.
Methods for Detecting Cell Adhesion
Cell-to-Cell Adhesion Analysis
In recent years, advances in microscopy techniques and biophysical measurements have opened the possibility to determine the biomechanical mechanisms behind the formation and function of intercellular contacts, cell migration and tissue remodeling. Different in vitro adhesion assays have been developed to characterize the strength of adhesion between cells.
- Flipping Assay
Compared to single-cell assays, the flipping assay is a relatively rapid and reliable method to study the function of different adhesion molecules in mediating cell-cell adhesion in different in vitro cell systems without the need for extensive laboratory equipment.
Cell–cell adhesion flipping assay [1].
- Atomic Force Microscopy (AFM)-Based Single-cell Force Spectroscopy (SCFS)
This is an ultra-sensitive method for quantifying the cell adhesion of individual cells under physiological conditions. By varying the residence time, the progression from single receptor binding to co-receptor binding can be monitored at the force level and correlated with the establishment of overall adhesion. It is also possible to map the adhesion properties of different regions on tissue explants or cell monolayers.
AFM-based single-cell force spectroscopy [1].
- Dual Micropipette Aspiration
Micropipette-based assays are an alternative method for quantifying cell-cell adhesion forces. BFP is an ultrasensitive force measurement device and provides unique information on the molecular mechanisms of single receptor-ligand interactions, but it has less application in the study of developmentally relevant processes that occur primarily at the cellular and tissue scale.
Dual micropipette assay [1].
Single Cell Adhesion Assay
Many methods have been established in the study of cell-matrix interactions, and many of these measurements are based on AFM (atomic force microscopy), which has become a common device for measuring intercellular forces because of its ability to accurately quantify cell-matrix adhesions.
Our Services
Research has shown that both internal and external physical forces can act through the cytoskeleton to affect local mechanical properties and cellular behavior. CD BioSciences has a well-established technology platform and sophisticated instrumentation to accurately measure the mechanical properties of cells.
- Cell Adhesion Analysis
- Cell-to-Cell Adhesion Analysis
- Cell-ECM Adhesion
- Cancer Cell Adhesion Analysis
- Cancer Cells Adhesion Analysis
- Effect of Drugs on Cancer Cells Adhesion
We have extensive experience in cell mechanics' research; we have been focusing on providing a high-quality biomechanical research platform and providing our customers with various cell mechanical properties testing services. For more information about our services, please feel free to contact us.
Reference
- Kashef J, et al. Quantitative methods for analyzing cell–cell adhesion in development[J]. Developmental biology, 2015, 401(1): 165-174.
For research use only. Not intended for any clinical use.
