Cat No. CA-11083
Product Size
Online Inquiry| Target Information | |
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| Synonyms | ACTB; Actin-Beta; Cytoplasmic 1 |
| Product Details | |
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| Product Name | Beta-Actin Rabbit Polyclonal Antibody |
| Description | Rabbit polyclonal antibody to beta-Actin. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Beta-actin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal region near amino acids 350-375 of human beta actin. |
| Conjugate | Unconjugated |
| Purification | Immunogen affinity purified. |
| Usage | |
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| Applications | WB, ELISA, EM, ICC/IF, IHC |
| Application Notes | Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at 42 kDa in size corresponding to beta actin by WB in the appropriate cell lysate or extract.Use in electron microscopy reported in scientific literature. |
| Dilutions | ELISA 1:10000-1:40000, ICC/IF 1:500-1:2000, IHC 1:10-1:500, WB 1:1000-1:4000. |
| Reactivity | Hu/Mu, Rt, Am |
| Molecular Weight | 42 kDa |
| Storage & Handling | |
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| Storage | Store at -20 °C. Avoid freeze-thaw cycles. |
| FormuLation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 0.01% Sodium Azide. |
| Disclaimer | This product is for research use only. It is not intended for diagnostic or therapeutic use. |
For research use only. Not intended for any clinical use.