Cat No. CA-11077
Product Size
Online Inquiry| Target Information | |
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| Synonyms | ACTB; Actin-Beta; Cytoplasmic 1 |
| Product Details | |
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| Product Name | Beta-Actin Mouse Monoclonal Antibody |
| Description | Mouse Monoclonal antibody (AC-15) to beta Actin. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | AC-15 |
| Isotype | IgG1 |
| Immunogen | This beta-actin antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
| Conjugate | Unconjugated |
| Purification | Unpurified. |
| Usage | |
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| Applications | WB, SimpleWestern, ELISA, FCM, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KO |
| Application Notes | Simple western reported by an internal validation. Separated by size-all, antibody dilution of 1:25-1:100; matrix was 12-230 kDa. |
| Reactivity | Hu/Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp,Ha, Le/ma, Pm, Rb, Sh, Sq, Tr, Ze, Dr(-) |
| Molecular Weight | 42 kDa |
| Specificity | In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixing and paraffin-embedding. |
| Storage & Handling | |
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| Storage | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
| FormuLation | Ascites 15 mM Sodium Azide. |
| Disclaimer | This product is for research use only. It is not intended for diagnostic or therapeutic use. |
For research use only. Not intended for any clinical use.