Microtubules play a key role in mitosis, intracellular transport and motility, and are a major therapeutic target in cancer. Also, microtubules are important for the targeted intracellular transport of vesicles, proteins and messenger RNAs. Immunohistochemical images show that many key cancer proteins are associated with microtubules. The specificity of monoclonal antibodies represents a potential therapeutic advantage, but their use as single agents in oncology has so far proved limited, but they remain an essential part of oncology research. CD BioSciences has become a trusted and reliable supplier to many leading international research institutions and clinical diagnostic companies by providing quality products to scientists in the life sciences worldwide. Our antibodies combine specificity, sensitivity and reproducibility to provide high quality antibody reagents for cytoskeleton research.
Different Types of Microtubule Subunits
As housekeeping genes, microtubule proteins are abundantly expressed in all cell types. Therefore, they are often used as internal reference controls in protein immunoblotting experiments. They are also commonly used as marker proteins to visualize microtubule networks in immunocytochemistry (ICC) and immunohistochemistry (IHC / IHC-P).
| Microtubule Subunits |
Description |
Human Microtubule Isoforms |
| α-Microtubule |
As a subunit of microtubule assembly, the α-subunit consists of 450 amino acids. To form microtubules, dimers of α- and β-microtubule bind to GTP and assemble to the (+) end of the microtubule in the GTP-bound state, where the α-microtubule subunit is exposed at the negative side. |
TUBA1A, TUBA1B, TUBA1C, TUBA3C, TUBA3D, TUBA3E, TUBA4A, TUBA8 |
| β-Microtubule |
The β-subunit is composed of 455 amino acids and their molecular weight is about 55 kDa. To form microtubules, the dimer of α- and β-microtubule binds to GTP and assembles to the (+) end of the microtubule in the GTP-bound state, and the β-microtubule subunit is exposed at the positive end of the microtubule. |
TUBB, TUBB1, TUBB2A, TUBB2B, TUBB2C, TUBB3, TUBB4, TUBB4Q, TUBB6 |
| γ-Microtubule |
γ-microtubule is another member of the microtubule protein family that is important in the nucleation and polar orientation of microtubules, and it is mainly found in centrosomes and spindle polar bodies. |
TUBG1, TUBG2 |
Our Services
CD BioSciences offers a wide range of monoclonal, polyclonal and recombinant antibodies against microtubule proteins. We are dedicated to the development and preparation of antibodies for various applications, providing high quality antibody products and R&D services to research institutes and pharmaceutical companies.
- Monoclonal and Polyclonal Antibody Development for Microtubules
The use of polyclonal and monoclonal antibodies has increased significantly in recent years. Each type of antibody has its own advantages and disadvantages, and we offer efficient antibody production services, so you can choose according to your research needs.
|
Monoclonal Antibodies |
Polyclonal Antibodies |
| Preparation Cost |
*** |
* |
| Preparation Speed |
* |
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| Non-specific Antibodies |
* |
*** |
| Recognition Sites |
Single Table Location |
Multiple Table Locations |
| Batch Differences |
* |
*** |
| Stabilization |
* |
*** |
| Advantages |
Commonly used to detect mixed proteins with high specificity. |
Usually preferred for detection of denatured proteins with higher affinity. |
- Model Organism Microtubule Antibody Development
We develop and produces cytoskeletal antibodies for a wide range of model organisms, providing researchers with antibodies to model organism cytoskeletal targets, including human, monkey, murine, rabbit, guinea pig, zebrafish, Drosophila, and C. elegans.
- Microtubule Antibody Development from Different Hosts
Mice and rats are the host species most commonly used to produce research antibodies, especially in the case of monoclonal antibodies. Whereas polyclonal antibodies can be produced in mice or rats, they are usually made in larger animals, such as rabbits, goats, donkeys or sheep, from which it is easier to collect large amounts of serum.
※There are different subclasses of IgG prepared using different species of animals, and the common subclasses of IgG from different species are as follows.
| Species |
IgG Subclass |
| Rabbit |
IgG (no subclass) |
| Mouse |
IgG1, IgG2a, IgG2b, IgG2c, IgG3 |
| Rat |
IgG1, IgG2a, IgG2b, IgG2c, IgM |
| Goat |
IgG1, IgG2 |
| Caw |
IgG1, IgG2 |
| Horse |
IgGa, IgGb, IgGc, IgG(B), IgG(T) |
| Pig |
IgG1, IgG2a, IgG2b, IgG3, IgG4 |
We provide antibody development services for different hosts (mouse, rat, rabbit, goat, etc.) according to the needs of different customers and are committed to fulfill your experimental needs in all aspects.
Antibody Customization Process
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
For research use only. Not intended for any clinical use.
