| Applications |
WB, Simple Western, FCM, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready |
| Application Notes |
This alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, IHC on paraffin-embedded and frozen sections, Immunocytochemistry/IF and Flow Cytometry.The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining. Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue; matrix was 12-230 kDa. Only 10 - 15 μL of the recommended dilution is used per data point. |
| Dilutions |
IF 1:50000-1:100000, IHC 1:100-1:500, IHC-Frozen 1:100-1:500, IHC-Paraffin 1:100-1:500, IM/IP 1:50-1:100, Simple Western 1:50, WB 1:5000. |
| Reactivity |
Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, Gp, Gt, Ha, Pa, Pm, Pm, Rb, Xp, Ye |
| Molecular Weight |
50 kDa |
| Specificity |
This alpha tubulin antibody (DM1A) does not cross-react with beta tubulin. |
