Biochemical and Structural Biological Services for Liquid-Liquid Phase Separation

Biomolecular condensates and membraneless organelles are formed by liquid-liquid phase separation (LLPS) caused by the interaction of proteins and nucleic acids. As an ideal partner for biomolecular condensates, CD Biosciences provides customized biochemical and structural biological services for liquid-liquid phase separations to clients worldwide to analyze the structural biology of protein and RNA binding domains that undergo LLPS.

Introduction to The Structure of Biomolecular Condensates

Biochemical processes within cells occur in a complex environment that is highly crowded, heterogeneous and full of interfaces. Cell biology is closely linked to the compartmentalization and formation of various biomolecular condensates and membraneless organelles, droplets that form through tightly controlled and often reversible LLPS in response to changes in the cellular environment. LLPS are ubiquitous in biology, serving as subcellular compartments for the separation and delineation of important biomolecular condensates in a variety of biological processes. Many of the proteins found in these complex condensates are inherently disordered, and their lack of stable structure is critical for the reversible LLPS-driven formation of these droplets. The presence of a large number of intrinsically disordered proteins in biomolecular condensates is functional.

Fig. 1. Examples of biomolecular condensate architecture. (Fare C M, et al, 2021)

Customized Services

The biophysical services of liquid-liquid phase separation provide a mechanistic framework for theoretical concepts of the phase transition behavior of biomolecular condensates. Here, we focus on biochemical processes without membrane compartments by developing promising in vitro cohesive layer model systems to properly model partially compartmentalized cellular environments. These model systems can systematically help clients analyze the role of the spatiotemporal organization of biomolecules in controlling cellular biochemical processes and provide important insights into the development of functional artificial organelles and cells.

Biomolecular condensates are formed by LLPS of specific proteins and nucleic acids. Proteins and nucleic acids play a crucial role in LLPS and define the mechanical properties and functions of biomolecular cohesions. Based on the needs of our clients to understand the structure and function of biomolecular condensates, CD BioSciences provides comprehensive biochemical and structural biological services for liquid-liquid phase separation to systematically study their assembly, physicochemical properties and their impact on biochemical processes.

• Identification of Molecular Structure in Biomolecular Condensates
• Characterization of Membraneless Organelles
• Services for Protein Liquid-Liquid Phase Separation
• Services for RNA Liquid-Liquid Phase Separation

With years of experience in biomolecular condensates, CD BioSciences offers professional and cost-effective biochemical and structural biological services for liquid-liquid phase separations. We will help you analyze the main properties of proteins and RNAs for liquid-liquid phase separation, the importance of inherent disorder in phase separation, and the importance of RNA-protein and RNA-RNA interactions in phase separation. Each project will be individually designed to meet the client's requirements and described in a detailed project report. If you are interested in our services, please contact us.