Services for RNA Liquid-Liquid Phase Separation
Liquid-liquid phase separation (LLPS) of specific proteins and nucleic acids forms different biomolecular condensates. The characteristics of these condensates depend on the multivalent molecular interactions between the constituent proteins and nucleic acids. As the study of phase separation progressed, scientists discovered that RNA, especially long-stranded non-coding RNA (LncRNA), also plays an important regulatory role in phase separation. Phase separation has a bidirectional regulatory relationship with RNA. Non-coding RNAs usually recruit proteins as molecular scaffolds to drive phase separation. Droplet properties such as viscosity, size and spatial organization are regulated by RNA in a manner that depends on RNA sequence, length and structure. On the other hand, phase separation is also involved in RNA transcription, translocation, and metabolism. RNA-RNA interactions promote condensation, and RNA is even able to undergo LLPS in the absence of proteins.
Fig. 1. LncRNAs are involved in phase separation in nucleus. (Guo Q, et al., 2021)
Customized Services
RNA can both promote and disrupt phase separation, depending on the particular RNA and its concentration. Our scientists are very interested in the key role of RNA in biomolecular cohesion, focusing on RNA and RNA-protein interactions. As experts in the biochemical and structural biology of phase separation, CD BioSciences offers comprehensive liquid-liquid phase separation services for RNA to help you analyze the bidirectional regulation of phase separation and RNA.
Analysis of Protein-RNA Interactions Underlying Liquid-Liquid
We offer customized single-molecule fluorescence procedures to probe protein-RNA interactions under LLPS. In addition, we have advanced in situ single-molecule high-resolution localization and counting methods to accurately measure the interactions between phase separated RNA and proteins in living cells.
RNA-Driven Phase-Separated Biocondensates Formation
RNA affects droplet formation and properties through RNA-protein, RNA-RNA and protein-protein interactions. Our focus is on how the concentration, length, sequence and structure of RNA affect droplet formation and properties.
Structural Biology of RNA Binding Domains
We can help you analyze the protein-RNA interactions of common RNA binding domains. Such individual structural domains provide important insights into the protein-nucleic acid interactions behind LLPS.
RNA-Protein Dynamics and Biocondensates Properties
RNA-protein interactions are highly dynamic. We can examine protein-RNA dynamic interactions at high protein concentrations to analyze the importance of such interactions in the formation of liquid-like droplets.
Regulation of RNA by Phase Separation
The type and advanced structure of RNA can give specificity to phase separated droplets, while phase separation also regulates RNA. We can provide you with an analysis of the regulation of RNA transcription, localization and metabolism by phase separation.
We analyze the multivalency, affinity and dynamics of RNA-protein interactions at the molecular level in relation to the overall nature of the coalescence, and the regulation of RNA by LLPS. We also focus on how misregulation of RNA-protein interactions can lead to abnormal phase separation and disease studies. If you have any special requirements for our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Guo Q, Shi X, Wang X. (2021) RNA and liquid-liquid phase separation[J]. Non-coding RNA Research. 6(2): 92-99.
For research use only, not intended for any clinical use.
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