Analysis of Biomolecular Condensates in Plants
Unlike animals, plants have the unique ability to sustain growth and development. Inherent in nature, plants are also exposed to different environmental conditions, such as light, temperature, and pathogens, that can reshape their developmental activities. Depending on the developmental stage or environmental conditions, plants alter global gene expression through coordinated signals between membrane-bound chloroplasts and mitochondria, and the nucleus. In addition, various membrane-free compartments have been identified within plant cells involved in plant development and responses to different environmental conditions. The vast majority of studies examining the function of various biomolecular condensates and the underlying physical principles that control their formation have been performed in fungal or animal systems. Studies examining phase separation in these systems have led to many breakthroughs in understanding fundamental cellular processes and the underlying mechanisms that drive disease. In contrast, relatively few studies have examined the phase separation of biomolecular condensates in plant systems.
Fig. 1. Emerging roles of phase separation in higher plants. (Xu X, et al., 2021)
Customized Services
Our experts are very interested in biological phase separation of biomolecular condensates in plants and have been working on protein phase separation in plant biology.
As a leading provider of biomolecular condensates, CD BioSciences offers professional analytical services to unravel the complexity of biomolecular condensates and their functional significance in plant biology. By utilizing advanced imaging techniques, proteomic analysis, and molecular characterization, we can decipher the molecular features, regulatory mechanisms and biophysical principles of plant phase separation. In addition, our team is well-equipped to address the unique challenges posed by studying biomolecular condensates in plants using methods optimized in other systems.
We aim to uncover plant-specific condensates, unique protein-protein interactions, and the dynamic regulation of plant phase separation. Our services contribute not only to a deeper understanding of plant biology but also to the broader field of phase separation and its impact on biological fitness and robustness. Our services include but are not limited to:
Our Platforms for Studying Plant Biomolecular Condensates
- Tissue infiltration of Nicotiana benthamiana leaves and protoplast transient expression systems.
- Computational platforms, including tools for predicting protein information, essential disordered regions, and their behavior.
- Microscopy platforms, including delayed differential interference contrast (DIC) imaging, fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP), numerical and brightness (N&B) analysis, etc.
The analysis of biomolecular condensates in plants is an emerging field with great potential to unravel the complexity of cellular organization and function. CD BioSciences leverages our extensive expertise, state-of-the-art facilities, and collaborative approach to provide comprehensive analytical services for biomolecular condensates in plants. By delving into the role of phase separation, characterizing plant-specific condensates, and revealing new mechanisms and adaptations, we aim to contribute to a deeper understanding of plant biology and the broader field of phase separation. If you have any special requirements for our services, please feel free to contact us. We are looking forward to working together on your attractive projects.
Reference
- Xu X, Zheng C, Lu D, et al. (2021) Phase separation in plants: new insights into cellular compartmentalization[J]. Journal of Integrative Plant Biology. 63(11): 1835-1855.
For research use only, not intended for any clinical use.
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