Analysis of LLPS in Plant Post-Transcriptional Processes

Plant communities can regulate plant growth and developmental processes through liquid-liquid phase separation (LLPS). CD BioSciences is committed to studying the emerging role of plant phase separation with the aim to provide comprehensive analytical services to explore LLPS in plant post-transcriptional processes.

Introduction to LLPS in Plant Post-Transcriptional Processes

Post-transcriptional regulation is one of the characteristics of eukaryotic gene expression, including a series of modifications and processing of transcripts, such as pre-mRNA 5'-end capping, splicing and 3'-terminal polyadenylation, RNA editing, RNA decay, etc. Studies have shown that LLPS is also an important regulatory mechanism in plant post-transcriptional processes. The RNA-binding protein FLOWERING CONTROL LOCUS A (FCA), which regulates Arabidopsis flowering, can form subnuclear condensates with liquid-like properties and undergo LLPS in vitro. EMBRYO DEFECTIVE (EMB1579) condensates sense endogenous and environmental factors to precisely regulate gene transcription, mRNA splicing, and plant development. LLPS is also involved in Arabidopsis mRNA N⁶-methyladenosine (m⁶A) modification.

Fig. 1. Biomolecular condensates formed by phase separation in the chromatin- and small RNA-related pathways. (Lei Z, et al., 2021)

Customized Services

CD BioSciences, a biotechnology company specializing in biomolecular condensates, provides comprehensive characterization services for LLPS analysis in post-transcriptional processes in plants. Through advanced technology and state-of-the-art equipment, our experts are dedicated to improving our clients' understanding of plant LLPS and its impact on the regulation of gene expression.

We offer integrated cellular biochemical and biophysical approaches to analyze the phase separation of biomolecular condensates during post-transcriptional processes in plants. These biomolecular condensates include FCA, FLX-LIKE 2 (FLL2), EMB1579, microRNA (miRNA), m⁶A, etc.

Our services include but are not limited to:

• Microscopic Analysis
  We offer advanced high-resolution imaging techniques such as confocal microscopy and super-resolution microscopy to visualize and analyze the formation and kinetics of liquid-like condensates involved in post-transcriptional processes in plants.
• Biochemical Analysis
  We offer a wide range of biochemical analysis services, including the characterization of prion-like structural domains (PrLD), intrinsically disordered regions (IDR), and low-complexity structural domains (LCD) in LLPS-associated proteins. Our biochemical techniques, such as circular dichroism and size-exclusion chromatography, are widely used to determine protein structure, conformational changes, and interactions critical to LLPS in post-transcriptional processes in plants.
• Functional Studies of Regulatory Factors
  We conduct functional studies to investigate the role of LLPS regulators in post-transcriptional processes in plants. Through genetic screens and manipulation of key regulators such as RNA binding proteins, convoluted helix proteins, and chromatin modifiers, we can help you elucidate the functional significance of LLPS in specific post-transcriptional events such as polyadenylation and small RNA pathways.
• Computational Modeling and Data Analysis
  In addition to experimental approaches, we utilize advanced computational modeling and data analysis techniques to gain insight into LLPS during post-transcriptional processes in plants. Molecular dynamics simulations and bioinformatics analysis provide valuable information about the behavior, interactions, and phase behavior of biomolecular condensates. These computational tools enhance our understanding of the physical principles behind LLPS and help interpret experimental results.

CD BioSciences draws on its extensive experience and cutting-edge technology to analyze the regulatory role of LLPS in plant gene expression. Through these efforts, we are paving the way for a deeper understanding of the dynamic properties of plant cells and their intricate post-transcriptional processes. If you have any special requirements for our services, please feel free to contact us.