Lysine-based VLP Modification Service

Lysine-based VLP Modification Service

CD BioSciences is a leading global provider of custom services, working closely with clients and experts in the construction of chimeric virus-like particles (cVLPs) by chemical conjugation methods. Our experienced scientists specialize in VLPlant^TM platform and are happy to meet each specific client requirement to provide the most appropriate solution to provide you with high quality and on-time delivery of cVLPs construct results.

Lysine-based VLPs Modifications

The classical approach to form cVLPs by chemical conjugation is based on lysine modification-using a cross-linking agent that reacts with the nucleophilic amino acid side chain on the VLPs. Because lysine has a primary amine, it is easily modified. When the pH is between 7.2 and 9, the lysine residues exposed on the surface form amide bonds through the ester reaction of n-hydroxysuccinimide (NHS) (NHS can be released as part of the reaction).

Chemical cross-linking for chimeric VLP assembly. (Brune KD, et al., 2018)

Application of Lysine-based Modifications of VLPs

This lysine-based chemical conjugation modification has been used to embed transferrin in VLPs, allowing VLPs to cross the blood-brain barrier and opening up a new therapeutic avenue for the treatment of neurological disorders. In addition, peptides, proteins and fluorescent probes can also be embedded in VLPs via the NHS reaction, resulting in cVLPs.

Our Services

As the world's leading custom service provider, CD BioSciences provides customers with VLP modification services based on cysteine-modified chemical conjugation methods.

Why Choose CD BioSciences

Experienced
We have a long history of providing VLPs modification services to our global clients and are adept at solving the difficulties and challenges encountered during experiments.

Flexible
We are in constant contact with our customers to discuss issues encountered during the construction of cVLPs in a timely manner.

High Quality
We have the ability and confidence to employ the best experimental protocols to meet your needs for rapid and accurate construction of cVLPs.

If you are interested in our services, you can contact us for more details., we are happy to hear from you and look forward to working with you.

Reference

1. Brune KD.; et al., New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue. Front Immunol. 2018, 9:1-15.