γ-[(6-Azidohexyl)-imido]-ATP sodium salt

Synonyms (s): γ-[(6-Azidohexyl)-imido]-adenosine-5’-triphosphate sodium salt

Empirical Formula (Hill Notation): C₁₆H₂₈N₉O₁₂P₃ · xNa⁺

Size: 1 mg

Product Introduction

Molecular Weight	631.37 (free acid basis)
PubChem Substance ID	329769105
Assay	≥95% (HPLC)
Form	Powder
Reaction Suitability	Reaction type: click chemistry
Smiles String	O[C@@H]([C@H]1O)[C@@H](COP(O)(OP(O)(OP(NCCCCCCN=[N+]=[N-])(O)=O)=O)=O)O[C@@H]1N2C=NC3=C2N=CN=C3N
InChI	1S/C16H28N9O12P3/c17-14-11-15(20-8-19-14)25(9-21-11)16-13(27)12(26)10(35-16)7-34-39(30,31)37-40(32,33)36-38(28,29)23-6-4-2-1-3-5-22-24-18/h8-10,12-13,16,26-27H,1-7H2,(H,30,31)(H,32,33)(H2,17,19,20)(H2,23,28,29)/t10-,12-,13-,16?/m1/s1
InChI key	FZLLGYSKHRTLRX-AARXTDBFSA-N
Storage Temp.	-20 °C

Application

Lee, et al., reported a non-radioactive version of in vitro phosphorylation where γ-[(6-Azidohexyl)-imido]-ATP (compound 6) was successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, azide-modified protein substrate can be labeled with alkynes of biotin or fluorescent dyes using Cu(I)-catalyzed click chemistry or DBCO-containing biotin or fluorescent dyes using Cu(I)-free click chemistry.

Safety Information

Storage Class Code	13 - Non Combustible Solids
WGK	WGK 3

For research use only. Not for clinical use.