Characterization of Biomolecular Condensates

Biomolecular condensates are membrane-free assemblies formed of proteins and nucleic acids. The complexity and depth of questions regarding the nature and organization of biomolecular condensates is increasing. As an ideal partner for biomolecular condensates, CD BioSciences provides customized characterization of biomolecular condensates services to analyze the formation, structure, dynamics, composition, activity and function of biomolecular condensates for clients worldwide.

Biomolecular condensates are membrane-free subcellular compartments that play a plethora of important functions in signaling and storage. Biomolecular condensates contain concentrated liquid-like droplets formed by liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs), often involving nucleic acids and other biomolecules. However, the liquid-solid phase transition to irreversible solid-like aggregates has been found in a range of neurodegenerative diseases. Therefore, in vitro and in vivo characterization and quantitative biomolecular condensates studies are essential to understand the underlying molecular determinants of protein phase transitions.

Fig. 1. Examples of complex composition of biomolecular condensates. (Mitrea D M, et al, 2022)

Customized Services

Our experts study the composition, physical properties, biochemical functions, and biomolecular assembly of biomolecular condensates, and are commmitted to developing advanced phase separation assays and biophysical measurement experimental techniques to characterize biomolecular condensates. Our services in this area focus on identifying coalescents, understanding their physical properties and regulation, cohesion functions, and how they arise from molecular components.

We are proud to have innovative experimental methods, conceptual frameworks, and computational approaches to address the complex structural, dynamic, and organizational features associated with biomolecular condensates. Here, CD BioSciences offers comprehensive characterization services for biomolecular condensates.

• Characterization of Biomolecular Condensate Material Properties
  We offer an integrated multidisciplinary approach based on the analysis of the formation and action of biomolecular condensates, including computer, biophysical, genetic engineering, and structural methods. We can characterize and/or measure material properties of biocondensates, including concentration, diffusion coefficient, viscosity, elasticity, interfacial tension, etc.
• Construction of Biomolecular Condensate Phase Diagrams
  LLPS depends on a variety of factors, including protein concentration, temperature, ionic strength, type of salt, pH, presence of co-solvents, etc. We can help you construct phase diagrams by varying these conditions.
• Analysis of Thermoresponsive Phase Behavior of Biomolecular Condensates
  We provide a temperature-dependent coarse-grained model to directly probe the thermoresponsive responsive phase behavior of biomolecular condensates that explicitly represents amino acid sequences and explains the temperature-dependent solvent-mediated interactions of each amino acid.
• Biochemical Analysis of Biomolecular Condensate Interactions
  Biomolecular condensates perform a variety of protein functions. We provide high-throughput phase separation methods for simple, sensitive and efficient identification recognition and understanding of protein-protein interactions (PPI). And further high-throughput screening of PPI modulators in vitro and in vivo, respectively.
• Reconstitution Experiments of Biomolecular Condensate Intrinsically Disordered Proteins
  We offer comprehensive reconstitution experiments of biomolecular condensates intrinsically disordered proteins to analyze the binding interactions that support the assembly of P-bodies, germ granules, stress granules, and other cohesions formed in the cytoplasm.
• Analysis of Control Centers of Biomolecular Condensate Phase Separation
  Cells can regulate the assembly of biomolecular condensates in time and space by regulating phase separation on a faster time scale. We offer specialized services to analyze the control centers of biomolecular condensate phase separation, focusing on three key cellular mechanisms: membrane surfaces, active processes, and post-transcriptional translation.

With years of experience in biomolecular condensates, CD BioSciences offers professional and cost-effective characterization services for biomolecular condensates. We integrate complementary approaches to solve problems related to the structure, dynamics and biology of biomolecular condensates. Each project will be individually designed to meet the client's requirements and described in a detailed project report. If you are interested in our services, please contact us.