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Contact NowIn eukaryotic cells, many protein systems can spontaneously organize into macromolecular condensates formed by liquid-liquid phase separation (LLPS). These organelles without membranes provide a strategy for organizing cells without physical barriers while allowing for the dynamic, reactive organization of cells. With advances in fluorescence imaging and single-molecule microscopy techniques, the discovery of biomolecular condensates in bacteria and protists is rapidly increasing. The structure, function, and potential applications of these microbial condensates are currently receiving extensive attention. Scientists have been able to study the characteristics of LLPS in vitro and in vivo by utilizing the latest imaging, structural and computational methods. However, in-depth studies of LLPS in relatively small prokaryotic cells remain technically challenging, limiting our understanding of the molecular basis and biological functions of microbial compartmentalization.
Fig. 1. Proposed phase transitions in bacterial cells. (Sołtys K, et al., 2022)
Studies have shown that bacterial cytoplasm displays glass-forming fluid properties and can solidify into soft glass-like forms, depending on metabolism, composition size, and non-site blocking interactions. Our lab has cutting-edge modern microscopy techniques to analyze biomolecular condensates in bacteria and protists, including the formation of phase-separated condensates in E. coli, Bacillus subtilis, Bacillus crescentus, etc. Our experts are committed to analyzing their biogenesis and biological function. In addition, we are exploring the molecular syntax of these biomolecular condensates to rationalize the design of synthetic condensates in mammalian cells.
CD BioSciences offers comprehensive biomolecular condensate analysis services in microbial cells to test the breadth of bacterial utilization of LLPS, examine its impact on enzyme kinetics and design synthetic membrane-free organelles. Our lab can characterize more than 10 bacterial LLPS systems, as following:
LLPS Systems in Prokaryotic Microbes | Representative Species |
---|---|
Polar organizing protein Z (PopZ) | Caulobacter crescentus |
Bacterial ribonucleoprotein bodies (BR-bodies) | Caulobacter crescentus |
Filamentous temperature-sensitive Z (FtsZ) | Escherichia coli |
Carboxysomes | Synechococcus elongatus |
ParABS system | Escherichia coli |
RNA polymerase (RNAP) | Escherichia coli |
Single-stranded DNA-binding protein (SSB) | Escherichia coli |
ATP-binding cassette transporters | Mycobacterium tuberculosis |
Polyphosphate granules | Pseudomonas aeruginosa |
DNA-binding protein from starved cells (Dps) | Escherichia coli |
We offer super-resolution imaging or infinite diffraction display combined with single molecule trafficking methods for the study of cells in LLPS and liquid condensates in prokaryotic systems, allowing for quantitative analysis.
CD BioSciences aims to help customers characterize biomolecular condensates in microorganisms, and analyze their formation and organization. Our services will open up more possibilities for future drug discovery. If you have any special requirements for our services, please feel free to contact us. We are looking forward to working together on your attractive projects.
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CD BioSciences is a company conducting biomolecular condensates targeted innovative drugs. We integrate the latest advances in physics, chemistry, biology, and machine learning to address some of the most fundamental challenges in health and disease today.
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