Genetics
Gene Symbol
Tg(CAG-EGFP/CETN2)3-4Jgg
Gene Name
transgene insertion 3-4
Site of Expression
GFP is expressed in a centrioles of the centrosome in every tissue examined from E14.5 through adult.
Expressed Gene(s)
CETN2, centrin 2, human; GFP, Green Fluorescent Protein
Strain of Origin
BALB/c x C57BL/6
Molecular Note
The GFP-CETN2 transgene was designed with a chicken beta-actin promoter (with cytomegalovirus immediate early enhancer and beta-actin intron) upstream of an EGFP-centrin 2 fusion protein (containing the full length human centrin 2 cDNA cloned in-frame into the C-terminal of enhanced green fluorescent protein), and followed by a beta-globin polyA sequence. Two lines were maintained (18-1 and 3-4) and line 3-4 was donated. These lines show consistent, robust expression of GFP in centrosomes (particularly in cerebellar granule cells).
Allele Symbol
Tg(CAG-Arl13b/mCherry)1Kvand
Gene Symbol
Tg(CAG-Arl13b/mCherry)1Kvand
Gene Name
transgene insertion 1
Site of Expression
Primary cilia on cells derived from the epiblast.
Expressed Gene(s)
RFP, Red Fluorescent Protein, coral; Arl13b, ADP-ribosylation factor-like 13B
Molecular Note
The transgenic construct is designed with a murine ADP-ribosylation factor-like 13B (Arl13b) cDNA fused in-frame upstream of a monomeric red fluorescent protein, mCherry, and driven by the pCAGGs promoter (which contains a chicken beta-actin promoter and CMV immediate early enhancer). Four founder lines were identified, only one exhibited consistent transgene expression.
Husbandry
Suggested Controls
C57BL/6J
Breeding Considerations
Double homozygous mice are viable and fertile. In 2019 colonists noted that double homozygous mice develop ear, eye, and nose sores by 12-14 weeks of age. Therefore, our colony is maintained by breeding females homozygous for the ARL13B-mCherry transgene and hemizygous for the Centrin 2-GFP transgene (hom hemi) with males hemizygous for the ARL13B-mCherry transgene and homozygous for the Centrin 2-GFP transgene (hemi hom).
Breeding Strategy
Hom for Tg(CAG-Arl13b/mCherry)1Kvand, hom for Tg(CAG-EGFP/CETN2)3-4Jgg x Hom for Tg(CAG-Arl13b/mCherry)1Kvand, hom for Tg(CAG-EGFP/CETN2)3-4Jgg
