Recombinant Human BRAF Protein

Cat. No.: CLPP-00150334

Product Size: 10 µg Custom size

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Product Overview

Description

CLPP-00150334 is recombinant human BRAF protein, mutated G466Y

Purity

> 80%

Applications

SDS-PAGE

Protein Length

Protein fragment

Animal Free

Nature

Recombinant Protein

Species

Human

Form

Liquid

Sequence

DLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSYSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH

Sequence Similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 RBD (Ras-binding) domain.

Predicted Molecular Weight

69 kDa including tags

Tags

GST tag N-Terminus

Target Information

Protein Name

BRAF

UniProt No.

P15056

Alternative Names

FLJ95109; 94 kDa B raf protein; B raf; B raf 1; B Raf proto oncogene serine threonine protein kinase; B Raf proto oncogene, serine/threonine kinase; B RAF1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF; BRAF 1; BRAF_HUMAN; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; Oncogen BRAF; oncogene BRAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; Raf; RAFB 1; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v raf murine sarcoma viral oncogene homolog B; v raf murine sarcoma viral oncogene homolog B1; v-Raf murine sarcoma viral oncogene homolog B1

Protein Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons.

Tissue Specificity

Brain and testis.

Involvement in Disease

Defects in BRAF are found in a wide range of cancers.Defects in BRAF may be a cause of colorectal cancer (CRC), lung cancer (LNCR), non-Hodgkin lymphoma (NHL), cardiofaciocutaneous syndrome (CFC syndrome), Noonan syndrome type 7 (NS7), LEOPARD syndrome type 3 (LEOPARD3).

Shipping & Handling

pH: 7.5

Constituents

0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine).

Shipping

Shipped on Dry Ice.

Storage

Store at -80 °C.

For Research Use Only. Not For Clinical Use.