Product Overview
Reactivity
Human, Mouse, Rat
Applications
ICC/IF, IHC-P, WB
Target Information
Alternative Names
Beta homolg of S. cerevisiae BUB 1; Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles; BUB 1B; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; Bub1A; BUB1B; BUB1B_HUMAN; BUB1beta; BUBR1; Budding Uninhibited by Benzimidazoles 1 beta; Budding uninhibited by benzimidazoles 1 homolog beta (yeast); hBUBR1; MAD3/BUB1 related protein kinase; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint gene BUB1B; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine protein kinase BUB1 beta; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; OTTHUMP00000160319; Protein SSK1; SSK 1; SSK1
Function
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Tissue Specificity
Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
Shipping & Handling
Shipping
Shipped on dry ice.
Storage
Store at 4 °C. Do Not Freeze.
Storage Buffer
PBS. pH: 7.2.
