p53 Nuclear Translocation Cell-based Assay Kit

Cat. No.
CLEK-00530376
Product Size
50 tests Custom Size

Product Information

Reactivity
Mammals
Sample Type
Adherent Cells, Suspension Cells
Assay Type
Cell-based
Detection Method
Flow cytometry-fluorescent
Form
96-Well Strip Plate

Target Information

Gene ID
Function
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Sequence Similarities
Belongs to the p53 family.
Alternative Names
Antigen NY-CO-13; Cellular tumor antigen p53; p53 tumor suppressor; P53_HUMAN; Phosphoprotein p53; Tp53; transformation related protein 53; tumor protein p53; Tumor suppressor p53; tumor suppressor TP53
Tissue Specificity
Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

Shipping & Handling

Shipping
Shipped on dry ice.
Storage Instructions
Store at -20 °C. Please refer to protocols.

For Research Use Only. Not For Clinical Use.

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