Product Overview
Applications
ICC/IF, IHC-P, WB
Target Information
Alternative Names
HSPK 21; HsPK21; NEK 2; NEK2; NEK2_HUMAN; NEK2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a) related kinase 2; NimA like protein kinase 1; NIMA related kinase 2; NimA related protein kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK 1; NLK1; Serine/threonine-protein kinase Nek2
Function
Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
Tissue Specificity
Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
Shipping & Handling
Shipping
Shipped on dry ice.
Storage
Store at 4 °C short term (1-2 weeks). Upon delivery aliquot. Store at -20 °C long term. Avoid freeze / thaw cycle.
Storage Buffer
78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine). pH: 7.00.
Preservative
0.01% Thimerosal (merthiolate)
