Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 16 bp deletion; Frameshift = 99.96%
Knockout Validation
Next Generation Sequencing (NGS), Western Blot (WB)
Cell Line Properties
Tissue
Epidermoid carcinoma
Adherent / Suspension
Adherent
Target Information
Gene Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Sequence Similarities
Belongs to the vinculin/alpha-catenin family.
Post-translational Modifications
Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques. Aceylated; mainly by myristic acid but also small amount of palmitic acid.
Storage & Handling
Recommended Control
Human wild-type A-431 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
