Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Tissue
Acute monocytic leukemia
Adherent / Suspension
Suspension
Target Information
Gene Function
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Sequence Similarities
Contains 1 CH (calponin-homology) domain.Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 SH2 domain.Contains 2 SH3 domains.
Post-translational Modifications
Phosphorylated on tyrosine residues.
Storage & Handling
Recommended Control
Human wild-type THP-1 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS + 0.05 mM β-mercaptoethanol
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
