Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and 1 bp insertion in exon 1 and insertion of the selection cassette in exon 1.
Knockout Validation
Sanger Sequencing
Cell Line Properties
Target Information
Gene Function
Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA.
Sequence Similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. Contains 1 RRM (RNA recognition motif) domain.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.