Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, insertion of the selection cassette in exon 1.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Target Information
Gene Function
It is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
Sequence Similarities
Contains 1 B box-type zinc finger.
Post-translational Modifications
Constitutively phosphorylated by PKC on serine/threonine in A431 cells.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
