Human TRIB3 Knockout HCT116 Cell Line

Cat. No.
CLCP-00350406
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
HCT116
Biosafety Level
BSL-1
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 35 bp deletion in exon 2
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Tissue
Colon carcinoma
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Male

Target Information

Gene Name
TRIB3
Gene ID
UniProt No.
Gene Function
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
Sequence Similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.Contains 1 protein kinase domain.

Storage & Handling

Recommended Control
Human wild-type HCT116 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
McCoY5a + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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