Human TPM3 (Tropomyosin 3) Knockout HEK293T Cell Lysate

Cat. No.
CLCP-00350666
Product Size
1 Kit

Product Overview

Parental Cell Line
HEK293T
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, homozygous: 1 bp insertion in exon 2.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
WB

Cell Line Properties

Tissue
Kidney
Morphology
Epithelial
Gender
Female
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
TPM3
Gene ID
UniProt No.
Gene Function
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Sequence Similarities
Belongs to the tropomyosin family.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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