Human TPM1 Knockout A549 Cell Line

Inquiry

Cat. No.

CLCP-00350398

Product Size

1 x 10⁶ cells/vial, 1 mL

Product Overview

Parental Cell Line

A549

Biosafety Level

BSL-1

Species

Human

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue

Lung carcinoma

Morphology

Epithelial

Viability

~80%

Adherent / Suspension

Adherent

Gender

Male

Target Information

Gene Name

TPM1

Gene ID

7168

UniProt No.

P09493

Gene Function

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Sequence Similarities

Belongs to the tropomyosin family.

Storage & Handling

Recommended Control

Human wild-type A549 cell line.

Cryopreservation Cell Medium

Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture Medium

F-12K + 10% FBS

Storage Instructions

Shipped on dry ice. Store in liquid nitrogen.

Storage Buffer

Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.