Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Cell Line Properties
Tissue
Acute monocytic leukemia
Adherent / Suspension
Suspension
Target Information
Gene Function
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
Sequence Similarities
Belongs to the p53 family.Contains 1 SAM (sterile alpha motif) domain.
Post-translational Modifications
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Higher levels of phosphorylation seen in the brain from patients with Huntington disease. Ubiquitinated; leading to its degradation by the proteasome.
Storage & Handling
Recommended Control
Human wild-type THP-1 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS + 0.05 mM β-mercaptoethanol
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
