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Human TP73 Knockout Raji Cell Line

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Cat. No.
CLCP-00350394
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
Raji
Biosafety Level
BSL-2
Species
Human
Knockout Validation
Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue
Burkitt's lymphoma
Morphology
Lymphoblastoid
Viability
~80%
Adherent / Suspension
Suspension
Gender
Male

Target Information

Gene Name
TP73
Gene ID
7161
UniProt No.
O15350
Gene Function
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
Sequence Similarities
Belongs to the p53 family.Contains 1 SAM (sterile alpha motif) domain.
Post-translational Modifications
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Higher levels of phosphorylation seen in the brain from patients with Huntington disease. Ubiquitinated; leading to its degradation by the proteasome.

Storage & Handling

Recommended Control
Human wild-type Raji cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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