Human TLN1 Knockout THP-1 Cell Line

Cat. No.
CLCP-00350381
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
THP-1
Biosafety Level
BSL-1
Species
Human
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Acute monocytic leukemia
Morphology
Monocytic
Viability
~50%
Adherent / Suspension
Suspension
Gender
Male

Target Information

Gene Name
TLN1
Gene ID
UniProt No.
Gene Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
Sequence Similarities
Contains 1 FERM domain.Contains 1 I/LWEQ domain.

Storage & Handling

Recommended Control
Human wild-type THP-1 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS + 0.05 mM β-mercaptoethanol
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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