Product Size
1 x 106 cells/vial, 1 mL
Product Overview
Parental Cell Line
Jurkat
Mutation Description
Knockout achieved by CRISPR/Cas9; X = 2 bp insertion; Frameshift: 100%
Knockout Validation
Next Generation Sequencing (NGS)
Cell Line Properties
Adherent / Suspension
Suspension
Target Information
Gene Function
Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.
Sequence Similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational Modifications
Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia. Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells.
Storage & Handling
Recommended Control
Human wild-type Jurkat cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.
