Product Overview
Parental Cell Line
HEK293T
Mutation Description
Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 3 and 8 bp deletion in exon 3.
Knockout Validation
Sanger Sequencing
Cell Line Properties
Target Information
Gene Function
Likely involved in the processes that promote cell division prior to the formation of differentiated tissues.
Sequence Similarities
Belongs to the TACC family. Contains 2 SPAZ (Ser/Pro-rich AZU-1) domains.
Post-translational Modifications
Isoform 1 is heavily phosphorylated; isoform 6 is not. Phosphorylated upon DNA damage, probably by ATM or ATR.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
