Human SORBS3 (Vinexin) Knockout HEK293T Cell Line

Inquiry

Cat. No.

CLCP-00350355

Product Size

1 x 10⁶ cells/vial, 1 mL

Product Overview

Parental Cell Line

HEK293T

Biosafety Level

BSL-2

Species

Human

Mutation Description

Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 13 and Insertion of the selection cassette in exon 13

Knockout Validation

Sanger Sequencing

Cell Line Properties

Tissue

Kidney

Morphology

Epithelial

Viability

~80%

Adherent / Suspension

Adherent

Gender

Female

Target Information

Gene Name

SORBS3

Gene ID

10174

UniProt No.

O60504

Gene Function

Vinexin alpha isoform promotes up-regulation of actin stress fiber formation. Vinexin beta isoform plays a role in cell spreading and enhances the activation of JNK/SAPK in response to EGF stimulation by using its third SH3 domain.

Storage & Handling

Recommended Control

Human wild-type HEK293T cell line.

Cryopreservation Cell Medium

Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture Medium

DMEM (High Glucose) + 10% FBS

Initial Handling Guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.

Storage Instructions

Shipped on dry ice. Store in liquid nitrogen.

Storage Buffer

Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.