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Human SIK1 Knockout HeLa Cell Line

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Cat. No.
CLCP-00350345
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
HeLa
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Cervix
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Female

Target Information

Gene Name
SIK1
Gene ID
102724428
UniProt No.
P57059
Gene Function
Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.
Sequence Similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.Contains 1 protein kinase domain.Contains 1 UBA domain.
Post-translational Modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.

Storage & Handling

Recommended Control
Human wild-type HeLa cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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