Product Overview
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2.
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Tested Applications
Sanger Sequencing, WB
Cell Line Properties
Target Information
Gene Function
Facilitates the differentiation and the cornification of keratinocytes.
Sequence Similarities
Belongs to the S-100 family. Contains 2 EF-hand domains.
Post-translational Modifications
Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.
Storage & Handling
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.
