Human RRAGC Knockout HeLa Cell Lysate

Cat. No.
CLCP-00350633
Product Size
1 Kit

Product Overview

Parental Cell Line
HeLa
Biosafety Level
BSL-2
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and insertion of the selection cassette in exon 1.
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Cervix
Morphology
Epithelial
Gender
Female
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
RRAGC
Gene ID
UniProt No.
Gene Function
Has guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Sequence Similarities
Belongs to the GTR/RAG GTP-binding protein family.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
DMEM (High Glucose) + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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