Human REC8 Knockout A549 Cell Lysate

Cat. No.
CLCP-00350628
Product Size
1 Kit

Product Overview

Parental Cell Line
A549
Biosafety Level
BSL-1
Species
Human
Mutation Description
Knockout achieved by using CRISPR/Cas9, 95 bp deletion in exon 6.
Knockout Validation
Sanger Sequencing

Cell Line Properties

Tissue
Lung carcinoma
Morphology
Epithelial
Gender
Male
Form
Liquid
Mycoplasma Free
Yes

Target Information

Gene Name
REC8
Gene ID
UniProt No.
Gene Function
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
Sequence Similarities
Belongs to the rad21 family.
Post-translational Modifications
Phosphorylated.

Storage & Handling

Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Initial Handling Guidelines
Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.
Storage Instructions
Store at -80 °C. Avoid freeze / thaw cycles.

For Research Use Only. Not For Clinical Use.

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