Human RBL2 Knockout THP-1 Cell Line

Cat. No.
CLCP-00350328
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
THP-1
Biosafety Level
BSL-1
Species
Human
Knockout Validation
Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue
Acute monocytic leukemia
Morphology
Monocytic
Viability
~80%
Adherent / Suspension
Suspension
Gender
Male

Target Information

Gene Name
RBL2
Gene ID
UniProt No.
Gene Function
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Sequence Similarities
Belongs to the retinoblastoma protein (RB) family.
Post-translational Modifications
During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.

Storage & Handling

Recommended Control
Human wild-type THP-1 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
RPMI + 10% FBS + 0.05 mM β-mercaptoethanol
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

Online Inquiry