Human PTCH1 Knockout A549 Cell Line

Cat. No.
CLCP-00350304
Product Size
1 x 106 cells/vial, 1 mL

Product Overview

Parental Cell Line
A549
Biosafety Level
BSL-1
Species
Human
Knockout Validation
Sanger Sequencing, Western Blot (WB)

Cell Line Properties

Tissue
Lung carcinoma
Morphology
Epithelial
Viability
~80%
Adherent / Suspension
Adherent
Gender
Male

Target Information

Gene Name
PTCH1
Gene ID
UniProt No.
Gene Function
Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
Sequence Similarities
Belongs to the patched family.Contains 1 SSD (sterol-sensing) domain.
Post-translational Modifications
Glycosylation is necessary for SHH binding.

Storage & Handling

Recommended Control
Human wild-type A549 cell line.
Cryopreservation Cell Medium
Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture Medium
F-12K + 10% FBS
Storage Instructions
Shipped on dry ice. Store in liquid nitrogen.
Storage Buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.

For Research Use Only. Not For Clinical Use.

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